EXPLICIT INPUT · TRIPLE-AXIS COUPLING

[ BIOPHYSICAL, BIOELECTRIC & SYSTEMIC PARAMETER CONSOLE ]

FAIL-CLOSED CONTRACT
1. LOCAL FRONT: TUMOR STROMA ACIDOSIS & NHE1 PROTON SHIELD (MET-01)
pH Scale (0.01 - 13.99)
Gated-6.50

pHe ≤ 6.50 induces unshielded pHi collapse & bioelectric acid trap.

molecules / cell

Target receptor threshold required for synapse engagement.

Nominal units (≥ 0)

ATP < 20 triggers immediate fail-closed metabolic veto.

MET-01 Hardware Variant

1K3R4E sustains pHi > 6.69, preventing depolarization & Cx43 closure.

2. BIOELECTRIC GOVERNANCE: Vmem, CONNEXIN GATING & COGNITIVE LIGHT CONE (LEVIN FRAMEWORK)
LIVE BIOELECTRIC CONNECTOR TELEMETRY (MET-01 → Vmem → Cx43 GGJ → CLC) INTEGRATED SELF (CLC = 0.911)
Cytosolic pHi (MET-01) 6.70
Membrane Potential (Vmem) -60.17 mV
Cx43 Conductance (GGJ) 0.911
Cognitive Light Cone (CLC) 0.911 (Open)
3. SYSTEMIC FRONT: MUCOSAL BARRIER INTEGRITY & ENTERO-HEPATIC AXIS
92.0%
0% (Leaky Gut) 50% (Endotoxemia) 90% (Rescue Gate) 100% (Akkermansia)
OPTIMAL EUBIOSIS (Akkermansia Active φ ≥ 90%)
ESTIMATED PORTAL & EPIGENETIC TELEMETRY (LIVE)
Portal IL-6 68.6 pg/mL
Tumor PD-L1 2.6x
H3K27me3 Silencing 18.2%
Active Lytic Time (ACT) 9.04 Hours

PARAMETERS READY FOR DETERMINISTIC EVALUATION.

TEMPORAL DYNAMICS (48 HOURS)

[ LIVE KINETIC RESPONSE & BIOELECTRIC PHASE ]

Cytolytic / Normalization Efficacy (%) Bioelectric / Metabolic Viability (%) Epigenetic Silencing (TOX / HDAC1 %)
MODEL EFFICACY METRIC: 100.0%
ACTIVE FUNCTIONAL TIME (ACT): 9.04 Hours
SIMULATED STATE: INTEGRATED SELF // COMPLETE CLEARANCE
MET-01 × LEVIN BIOELECTRIC CONNECTOR (pHe = 6.20, q_met = 180 pmol/min)

[ COUPLED pHi, Vmem, Cx43 GATING & COGNITIVE LIGHT CONE DASHBOARD ]

OPEN FULL RESOLUTION ↗
MET-01 Bioelectric Connector 4-Panel Dashboard comparing S703A vs 1K3R4E under pHe = 6.20 stromal acidosis
GAME THEORY WITH SELF-SCALING PLAYERS (IPD-ms) × MET-01 COUPLING

[ SPATIAL MULTICELLULARITY, MERGE/SPLIT ACTIONS & BIOELECTRIC GATING ]

OPEN IPD-ms DASHBOARD ↗
FORMAL ACADEMIC ATTRIBUTION & THEORETICAL CITATION

This spatial module is directly based upon the self-scaling game-theoretic framework developed by:
Lakshwin Shreesha, Federico Pigozzi, Adam Goldstein, & Michael Levin (2025).
"Extending Iterated, Spatialized Prisoner's Dilemma to Understand Multicellularity: Game Theory With Self-Scaling Players".
IEEE Transactions on Molecular, Biological, and Multi-Scale Communications, Vol. 11, No. 2, pp. 101–115.
Allen Discovery Center at Tufts University & Wyss Institute for Biologically Inspired Engineering at Harvard University.

Biophysical Coupling (Guanes Health Layer B): Modulates the probability of the Merge (M) action by Connexin-43 Gap Junction Conductance G_GJ(ΔVmem, pHi) and triggers involuntary Split (S) events under deep stromal acidosis (pHe ≤ 6.20) when unshielded.

SPATIAL GRID (20×20 = 400 NODES) ROUND: 100 / 100
Isolated Unicells Small Clusters Large Syncytium
Autonomous Agents 51 / 400
Max Syncytium Size 39 Cells
Syncytial Fitness 29.79
Merge Physical Gating G_GJ = 0.911
4-Panel IPD-ms Self-Scaling Simulation Dashboard
LAYER B MODEL REPORT / IN SILICO

[ MODEL CONSTRAINT & BIOELECTRIC EVALUATION ]

[ SIMULATION OK // CONSTRAINTS SATISFIED ]

IN-SILICO TELEMETRY (ASSUMED PARAMETERS)

Simulation StatusCONSTRAINTS_SATISFIED
Extracellular pH (pHe)6.20
Cytosolic pHi (MET-01)6.697
Membrane Potential (Vmem)-60.17 mV (Hyperpolarized)
Cognitive Light Cone (CLC)0.911 (INTEGRATED SELF)
Proton Shield / NHE11K3R4E (Constitutive NHE1-Shield)
Mucosal Integrity (phi_gut)92.0% (Microbiome Proxy)
Biophysical InvariantsSATISFIED (Valid in-silico bounds)

TELEMETRY PAYLOAD (RAW IN SILICO JSON)


            

          

        

      


      
      

        

          
            [ PARAMETER EXTRACTION // EXPERIMENTAL PARSER ]
            Expand to evaluate unstructured text via strict contract extraction
          
          

            

              Extracts biophysical variables from text with FILL_POLICY = NEVER. Detected variables are checked against the deterministic contract prior to simulation.
            

            

              
              

            

            

              TEST CASES: